J. Anim Sci.
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J. Anim Sci. 1974. 39:720-724.
© 1974 American Society of Animal Science
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Measurement of Bovine Plasma Testosterone by Radioimmunoassay (RIA) and by a Rapid Competitive Protein Binding (CPB) Assay

Mordechai Shemesh and William Hansel

Cornell University1, Ithaca, New York

Abstract

Changes in the peripheral plasma level of testosterone during the bovine estrous cycle were determined by a rapid competitive protein-binding assay and by radioimmunoassay, using a specific antiserum against testosterone following chromatographic purification of the steroid on Sephadex LH-20 columns. Values obtained by the two methods were similar. Testosterone concentrations increased during the 3 days preceding estrus and attained peak values (47 ± 9 pg/ml) 10 to 16 hr. before the onset of estrus. Minor rises were observed on days 3 to 5 and 11 to 14. The levels of testosterone observed during the cycle generally paralleled the plasma levels of estrogen observed in previous studies.

Footnotes

1 Department of Animal Sciences, and Division of Biological Sciences.






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