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Reproductive Hormones in Blood and Urine of Ovariectomized Sows^{1, 2,}

Purdue University, West Lafayette, Indiana 47907

Abstract

Four yearling sows, averaging 170 kg body weight, were ovariectomized 14 days after estrus (Day 0) to study subsequent rates of depletion of ovarian steroids in blood and their metabolites in urine for a period of 35 days. Blood and urine were collected during estrus, 7 days after estrus, before (Day 0) and after (Day 0.1) ovariectomy, daily through Day 14, and then on Days 21, 28 and 35. Urine was collected from three other sows 245 days after ovariectomy. Plasma total progestin, cortisol and corticosterone were quantified by competitive protein binding and luteinizing hormone (LH) was measured by radioimmunoassay. Urinary estrone and four urinary metabolites of progesterone (pregnanes) were quantified by gas liquid chromatography. Plasma cortisol and corticosterone were significantly (P< .025) increased only at Day 0.1 after ovariectomy. Plasma LH levels increased (P< .005) by Day 1 and remained elevated through Day 35. Plasma progestin (ng/ml) decreased from 18.7 ± 6.3 ng/ml on Day 0 to 4.8 ± 1.9 on Day 1 and to 1.8 ± 0.2 on Day 2. Thereafter, no further decreases were detectable. In contrast, urinary pregnanes decreased only 50% by Day 1 and did not decrease to estrous levels until Days 5 to 6. Average daily excretion of total urinary pregnanes was 7.6 mg/day 14 days after estrus (Day 0) compared to 2.4 mg/day at estrus and 0.48 and 0.17 mg/day 35 and 245 days after ovariectomy, respectively. At day 245, 90% of the total pregnanes was 5-pregnan-3ß-ol-20-one. Average excretion of urinary estrone on Day 245 was about 14% (3 µ/day) of the Day O rate.

¹ Journal Paper No. 5401, Purdue Agricultural Experiment Station. A contribution from the Department of Animal Sciences.

² The authors thank Dr. G. D. Niswender, Department of Physiology, Colorado State University, Fort Collins, for measuring blood plasma LH.

³ Present address: Department of Population Dynamics, The Johns Hopkins University, 615 N. Wolfe Street, Baltimore, Maryland 21205.

⁴ Present address: U.S.D.A. Range Livestock Station, Miles City, Montana 53901.