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Radioimmunoassay of Porcine FSH^{1, 2,}

University of Maryland, College Park

Abstract

A radioimmunoassay for porcine FSH has been developed and validated. The iodinated antigen contained a contaminant that cross reacted with porcine serum. This contaminant was removed by rechromatography of the labeled antigen on a column containing a dowex resin that was equilibrated with porcine serum. Porcine PRL, ACTH and GH did not compete in the assay. On a mass basis, 125 to 150 times more porcine LH was required for equivalent displacement of ¹³¹I PFSH from the antibody. The biologic-radioimmunologic ratios of several crude pituitary extracts ranged from .45 to 1.33. The minimal sensitivity of the RIA was 2.5 ng PFSH LER 1132 (66-67) and the FSH in 500 ul or less of porcine serum or plasma could be measured accurately and precisely. The RIA was employed to determine the concentration of FSH in the serum of female swine during different experimental states.

¹ This investigation was supported by USPHS Research Grant HD 00739 from the National Institute of Child Health and Human Development to H.J.B., by Project C-34, Maryland Agric. Exp. Station to E.P.Y. and by USPHS Research Grant AM-3598 (NIAMDD) to L.E.R. Scientific Article No. A1925, Contribution No. 4847 of the Maryland Agric. Exp. Station, Department of Animal Science.

² The authors wish to express their deep appreciation to Drs. Griff T. Ross and Mortimer Lipsett for the use of their laboratory and for many helpful suggestions. We also would like to thank Mr. Rudolph Reid for his expert technical assistance.

³ Endocrinology service. Reproduction Research Branch, National Institute of Child Health and Human Development, Bethesda, Maryland. Present address: Department of Surgery, Medical Branch, University of Texas, Galveston, Texas 77550.

⁴ Department of Zoology, University of Maryland, College Park 20742.

⁵ Department of Animal Science, University of Maryland, College Park 20742.

⁶ Department of Biochemistry, Emory University, Atlanta, Georgia 30322.