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Sex Ratios of Calves from Inseminations after Electrophoresis of Sperm¹

Michigan State University, East Lansing 48824

Abstract

Sperm from four ejacula from each of four bulls were electrophoresed at 8 and 21 V/cm in 10% yolk-0.10M phosphate (pH 7.10) for 1 min. at 35°. After electrophoresis, semen from the anode and from the cathode was glycerolated, frozen and used for inseminations. Whether the sperm were taken from the anode or cathode did not influence significantly the percent male calves resulting from these inseminations; we found 55% males among 121 calves from anode sperm, 44% males among 137 calves from cathode sperm and 44% males among 50 calves from unelectrophoresed control sperm.

¹ Published with the approval of the Director of the Agricultural Experiment Station as Paper No. 6461. Select Sires, Inc., Columbus, Ohio generously provided semen for this research and James Day assisted in arranging inseminations through Michigan Animal Breeders Cooperative, East Lansing. This research was supported in part by the North Central Breeders Council and by The National Association of Animal Breeders.

² Department of Dairy Science.

³ Present address: Animal Science Division, University of Georgia, Athens 30602.