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A Simple Method of Preparation of Gastroinestinal Tract Tissues for Scanning Electron Microscopy¹

University of Maine at Orono, Orono 04473

Abstract

Equipment found in many animal science oriented laboratories was used to develop a reliable straight forward procedure for preparing animal soft tissue for scanning electron microscopy. Tissues were fixed in glutaraldehyde and osmium tetroxide, dehydrated in alcohol and dried in a lyophilizer. It was found that freezing was not essential for artifact-free appearance. Charging under the electron beam was further reduced by attaching specimens to SEM mounting stubs with the electrically-conducting cement cited herein. This procedure resulted in gastrointestinal tract surfaces which appeared as previously described by histologists and transmission electron microscopists.

¹ This work was funded by State project S-3096, Agway Inc., Syracuse, N.Y., and Hatch project 289 from the Maine Agricultural Experiment Station in Department of Animal and Veterinary Sciences.