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Acrosome Morphology of Boar Spermatozoa during in vitro Aging

U. S. Department of Agriculture^,2, Beltsville, Maryland 20705

Abstract

Changes in the acrosome morphology of boar spermatozoa during in vitro storage were assessed by phase-contrast microscopy. During storage of the spermatozoa at 15 and 25 C the apical ridges enlarged and their smooth crescent-shape became irregular. The acrosomes deteriorated more rapidly when stored at 25 C than at 15 C and deteriorated more rapidly in Tris and saline extenders than in citrate and IVT extenders.

Five semen extenders were compared for their ability to maintain sperm motility and acrosome morphology during in vitro storage for 3 days at 25 C and for 7 days at 15 C. Maintenance of acrosomes with normal apical ridges and sperm motility was similar for Beltsville LI (BLI), Purdue extender (PE), and CO₂-saturated-IVT-extended semen at both 15 and 25 C. Greater acrosome deterioration at 25 C and lower sperm motility at 15 and 25 C occurred in the semen extended in IVT without CO₂ saturation. The poorest preservation of acrosome morphology and sperm motility occurred in glucose-bicarbonate (GB) extended semen.

¹ The authors express appreciation to Dr. Truitt H. Brinsfield, Jr., for development of the photomicrographs.

² Animal Physiology and Genetics Institute, Agricultural Research Center, Agricultural Research Service.