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Observations on the Postmortem Histochemistry of Myofibers from Stress Susceptible Pigs^{1, 2,}

University of Wisconsin, Madison 53706

Abstract

Postmortem myofiber histochemistry was studied in longissimus muscles of pigs with differing degrees of stress susceptibility (SS). The histochemical reactivity of phosphorylase and the stainability of glycogen by the periodic acid-Schiff (PAS) reaction was enhanced in samples taken approximately 20 min. postmortem. In later postmortem samples from SS animals killed by stress treatment, phosphorylase and PAS reactions were lost in all except some red myofibers. Of 12 SS animals examined, seven had a high proportion of white myofibers, three had a high proportion of intermediate type myofibers and two had myofibers with a high glycogen content but no demonstrable phosphorylase activity. Extremely SS animals had more myofibers with a positive PAS reaction than moderately SS animals.

¹ Research supported by the College of Agricultural and Life Sciences, University of Wisconsin, Madison, and by Public Health Service Research Grant No. FD-00107-14. Muscle Biology Laboratory Manuscript Number 47.

² The study was conducted in collaboration with Dr. D. Marple and Dr. G. Eikelenboom. The help of Nancy E. Swatland is gratefully acknowledged.

³ This work was done during the tenure of a postdoctoral fellowship of the Muscular Dystrophy Associations of America.