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Effect of Holding Time on Storage of Boar Spermatozoa At 5 C

U.S. Department of Agriculture,¹ Beltsville, Maryland 20705

Abstract

Three experiments were conducted with boar spermatozoa to determine the effects of holding time, extender composition, and dilution rate on acrosome morphology and sperm motility during storage at 5 C.

Holding undiluted semen at room temperature (24 to 26 C) for 1.25 to 7.25 hr. before cooling improved the maintenance of sperm acrosome integrity and sperm motility during storage at 5 C when compared to semen held for 0.25 hr. before dilution and cooling. The optimum holding time appeared to be about 6 hours.

The egg-yolk-glucose-bicarbonate (EGB) extender maintained acrosome integrity and sperm motility more effectively than did the Beltsville L1 (BL1) or Beltsville L2 (BL2) extender. There were no consistent differences between dilution rates of 1:3 (initial volume: final volume) and 1:6 when semen was extended in EGB following holding for 4.25 to 7.25 hours. Extending the semen in EGB during the holding period did not improve the maintenance of sperm acrosome integrity and sperm motility during storage at 5 C.

¹ Animal Physiology and Genetics Institute, Agricultural Research Center, Agricultural Research Service.