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Amino Acid Oxidation and Plasma Amino Acid Levels in Sheep with Abomasal Infusions of Graded Amounts of Lysine^{1, 2,}

University of Illinois at Urbana-Champaign,⁵ Urbana 61801

Abstract

The potential of the oxidation technique for estimating the amino acid requirement of the sheep was investigated. A series of seven infusion trials was conducted in which a wether lamb, fed a low protein diet, was made limiting in lysine by the abomasal infusion of a mixture of the essential amino acids devoid of lysine. This limitation was sequentially removed in subsequent trials by the abomasal infusion of graded amounts of lysine. Plasma-free amino acid concentrations and the oxidation of lysine, monitored as expired radioactivity from the oxidation of arterially injected L-lysine-U-¹⁴C hydrochloride, were measured in all trials.

The production of ¹⁴CO₂ and the plasma-free lysine concentrations did not markedly increase until a certain level of abomasal lysine supplementation had been attained. This level was estimated to be 2.1 and 2.4 g of supplemental lysine using ¹⁴CO₂ production and plasma-free lysine levels as response criteria, respectively. The amount of lysine entering the abomasum from the rumen in this experiment was estimated to be 4.4 g per day. Thus, the abomasal lysine requirement for this sheep fed this particular diet and made limiting in lysine was estimated to be 6.5 to 6.8 g per day. The points at which plasma-free amino acid concentrations and carbon dioxide production from an amino acid begin to rapidly increase, provide similar estimates of the lysine requirement of the sheep.

¹ Supported in part by Federal Hatch 353.

² Data taken from thesis submitted by the senior author to the Graduate College, University of Illinois, in partial fulfilment of the requirements for the Ph.D. degree.

³ Present address: An foras Taluntais, Dunsinea, Castleknock, Co., Dublin, Ireland.

⁴ Present address: Smith Kline and French Laboratories, 1600 Paoli Pike, Westchester, Pa.

⁵ Animal Science Department.

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