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Radioimmunoassay of Ovine FSH

University of California, San Francisco 94122

Abstract

A Radioimmunoassay for ovine FSH has been developed. The antiserum utilized produced two precipitant lines in Ouchterlony diffusion plates, neutralized the biological activity of ovine and murine FSH but not HCG. A method is described for the determination of iodination efficiency and damage as well as purification of the hormone tracer. Ovine LH, ACTH, prolactin, GH and bovine TSH did not compete in the assay. Further specificity of the assay was determined by comparison of biological and immunological potencies of several ovine preparations. Minimal sensitivity of the assay was about 2 ng FSH (Papkoff-PAFI). The assay has been applied to the measurement of the disappearance rate (T) of an IV injection of ovine FSH into mature female rats.

¹ Submitted in partial fulfillment of Ph.D. program.

² The authors wish to thank NIH for the gift of purified ovine FSH-S-3 and Dr. H. Papkoff for purified ovine prolactin, FSH, LH, ACTH utilized in these studies. The purified bovine TSH was a gift from Dr. S. Kaplan. The thoughts of Drs. M. Grumbach, S. Kaplan, H. Papkoff and W. F. Ganong during the development of the assay were of great benefit. This study was supported by USPHS program Project Grant AM06704, NIH Training Grant #AM05613 of the National Institutes of Arthritis and Metabolic Diseases, University of California Breon Fund, and Simon Fund.