J. Anim Sci.
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J. Anim Sci. 1973. 36:749-753.
© 1973 American Society of Animal Science
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Difficulties Associated with the use of Tritiated Polyethylene Glycol as a Water-Soluble Digestion Marker in Ruminants1

T. S. Neudoerffer2, D. R. McLaughlin2, S. J. Slinger2 and F. D. Horney4

University of Guelph, Guelph, Ontario

Abstract

Tritiated polyethylene glycol was examined as a water-soluble intestional digestion marker in cattle. Poor and variable recoveries from the aqueous digesta phase was observed. In vivo and in vitro studies on the distribution of the marker among digestive fractions (aqueous and particulate) showed that marker association with the particulate fraction occurred. It is suggested that difficulties arise in the interpretation of results of the absorption from the aqueous phase because of the distribution of the marker between the two digestive phases.

Footnotes

1 Financial support of the Ministry of Agriculture and Food is acknowledged.

2 Department of Nutrition.

4 Department of Clinical Studies.






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Copyright © 1973 by the American Society of Animal Science.