J. Anim Sci.
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J. Anim Sci. 1973. 36:712-715.
© 1973 American Society of Animal Science
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Zeranol and Dietary Protein Level Effects on DNA, RNA and Protein Composition of Three Muscles and the Relationship to Serum Insulin and GH Levels of Steers1

M. L. Borger2, J. D. Sink, L. L. Wilson, J. H. Ziegler3 and S. L. Davis4

The Pennsylvania State University, University Park, Pennsylvania 16802

Abstract

The effects of zeranol and dietary protein level on DNA, myofibrillar, sarcoplasmic, non-protein, and stroma protein N concentrations in the longissimus, adductor and intercostal muscles of 36 steers were determined. Certain correlations of plasma GH and insulin levels with these characters were also determined. Nuclear Feulgen-DNA and sarcoplasmic Azure B-RNA values were obtained by standard plug microspectrophotometry.

Significantly (P<.01) lesser amounts of NPN and sarcoplasmic protein N and greater amounts of stroma protein N were observed in inter-costal vs. longissimus and adductor muscles. Also, inter-costal muscle averaged significantly greater for percent lipid and less for percent water than did either of the other muscles. Mean DNA values were depressed in inter-costal vs. adductor muscles while RNA values were elevated in inter-costal as compared to longissimus and adductor muscles.

The only significant treatment effect was an increased percent water and decreased percent lipid in longissimus samples of implanted steers, and a zeranol X; dietary protein level interaction (P<.05) on DNA values. The implanted 11% protein group had depressed DNA values as compared to all other groups except non-implanted 9.5 and 12.5% protein groups.

GH levels were significantly (P<.05) correlated at day 84 with RNA for inter-costal muscle (r=–.43), at day 139 with NPN of inter-costal muscle (r=0.37) and at day 169 with stroma protein N of inter-costal muscle (r=0.45).

Footnotes

1 Paper No. 4127 in the Journal Series of the Pennsylvania Agricultural Experiment Station.

2 Present address: Department of Clinical Pathology, Milton S. Hershey Medical Center, Hershey, Pennsylvania.

3 The authors express their appreciation to Commercial Solvents Corporation for partial financial support of this research.

4 Department of Clinical Pathology, Milton S. Hershey Medical Center, Hershey, Pennsylvania.






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Copyright © 1973 by the American Society of Animal Science.