J. Anim Sci.
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J. Anim Sci. 1973. 36:658-662.
© 1973 American Society of Animal Science
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Fat Mobilizing Lipase in Relation to Fatness in Lambs1

K. S. Sidhu2, R. S. Emery2, A. F. Parr3 and R. A. Merkel3, 4,

Michigan State University, East Lansing 48823

Abstract

Basal and potential fat mobilizing lipase assays were developed for ruminant adipose homogenates. Cyclic AMP (4 uM), ATP (40 µ(M) and MgCl2 (133 µM) as cofactors increased (P<.05) lipolysis from 1.0 to 1.8 umoles fatty acids released/hr/mg protein. Addition of cyclic AMP-dependent protein kinase did not increase lipolysis. Cyclic AMP caused an apparent increase (P~.25) in basal protein kinase activity of adipose homogenates from 6.5 to 8.6 mumoles 32P incorporated/hr./mg protein.

Fat mobilizing lipase activity was determined in subcutaneous adipose tissues of lambs differing in age (8, 16 and 32 weeks), sex (ewe, wether, and ram) and breed (Southdown and Suffolk sired lambs) (3 x 3 x 2 factorial, replicated once). Basal lipolytic activity at 8, 16, 32 weeks of age were 0.5, 0.6 and 1.8 units, respectively. Comparable lipolytic values with cyclic AMP activation (potential) were 0.8, 1.3 and 3.1. Fat mobilizing lipase activities were higher (P<.05) at 32 than at 8 or 16 weeks. Lambs sired by Southdown rams responded to age effect more than those sired by Suffolk rams. Influence of sex on basal and potential fat mobilizing lipase was small, but rams had low activities relative to ewes and wethers. Basal fat mobilizing lipase activity was higher (P<.01) in adipose tissues from Southdown sired lambs compared to those of Suffolk sired lambs (1.6 vs. 0.4), possibly due to a higher proportion of the active form of fat mobilizing lipase in lambs sired by Southdown rams than those sired by Suffolk rams. The difference between breeds for potential fat mobilizing lipase was not significant. Lipolysis appears to increase with fatness in lambs.

Footnotes

1 Michigan Agricultural Experiment Station Journal Article No. 5949. Partially supported by National Institutes of Health Grant AM 13177.

2 Department of Dairy Science.

3 Department of Animal Husbandry.

4 Department of Food Science and Human Nutrition.






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