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Zero Time Rate Technique for Evaluating Nitrogen Supplements for High Roughage Rations^{1, 2,}

Oklahoma State University,³ Stillwater 74074

Abstract

FOUR rations consisting of a weathered bermudagrass hay plus isonitrogenous supplements of (I) cottonseed meal (CSM), (II) biuret (B), (III) biuret+ground corn (GC) and (IV) urea (U)+CSM were fed to lambs to evaluate the rate of VFA production in vitro as supported by the four rations and to compare the results to those of classical digestion trials. The VFA production was measured by the zero time rate technique at 4 and 8 hr. postfeeding on days 34, 63 and 82 from the start of the feeding of the nitrogen supplements. Differences in the rate of VFA production between treatments were more evident at 4 hr. postfeeding than at 8 hr. postfeeding. The biuret-containing rations tended to support a greater rate of VFA production at 8 hr. than at 4 hr. postfeeding. The CSM and U+CSM rations supported higher rates of VFA production at 4 hr. postfeeding than the biuret rations, whereas, the rates at 8 hr. postfeeding were lowest for the CSM ration. The zero time rate technique appears to have an application in studies of N-supplementation of poor quality roughage rations, whereas, proof of adaptation to NPN sources could not be shown in accompanying digestion trials.

¹ Journal Article J-2444 of the Agricultural Experiment Station, Oklahoma State University, Stillwater.

² The partial support of this work by the Lone Star Gas Company, Dallas, Texas, is gratefully acknowledged.

³ Department of Animal Sciences and Industry.