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Effects of Dilution and Glycerol on the Release of Glutamic-Oxaloacetic Transaminase (GOT) from Boar Spermatozoa¹

University of Minnesota, St. Paul, Minnesota 55101

Abstract

Three trials were conducted to examine the effects of dilution and glycerol addition on boar spermatozoa utilizing increased extracellular GOT as a determinant of cellular damage. Both dilution of boar semen with Tris-tricine extender and glycerol addition resulted in increases in extracellular GOT. It was also demonstrated that glycerol damaged the spermatozoa regardless of when or how it was added, and the damage was proportional to the glycerol concentration. Dilution of glycerolated spermatozoa with glycerol-free extender resulted in further damage perhaps caused by lysis as demonstrated with bovine erythrocytes.

¹ Scientific Journal Article No. 7923. Minnesota Agricultural Experiment Station. This work was supported by Hill Family Foundation St. Paul, Minn. Grant.

² Present address: American Breeders Service Inc., DeForest, Wisconsin 53532.