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Metabolism of ¹⁴C-Diethylstilbestrol in Sheep

United States Department of Agriculture,³ Fargo, North Dakota 58102

Abstract

THE excretion and excretory products of oral diethylstilbestrol (DES) in sheep were studied using monoethyl-1-¹⁴C-diethylstilbestrol (¹⁴C-DES). Recovery of ¹⁴C in feces and urine from five of six sheep was quantitative (97 to 102%) within 7 days of ingestion. Feces were the major (84 to 95%) excretory route. No ¹⁴C was observed when the expired air from three of the sheep was monitored for ¹⁴CO₂. Level of intake (3 to 100 mg/day) or continuous ingestion of nonlabeled DES did not affect rate or route of excretion. The concentration of ¹⁴C in blood reached its maximum within 16 hr. and then declined rapidly to below measurable levels by 96 hr. after ingestion. Samples of various tissues and organs taken 7 days after ingestion (when sheep were sacrificed) did not contain measurable ¹⁴C, with the exception of the adrenal glands of three sheep. The ¹⁴C in feces was present as free DES. This was verified by chromatographic and spectral (infrared and mass) comparisons with authentic DES. No free PES was found in urine; however, urinary ¹⁴C was not fully characterized.

¹ Animal Science Research Division, A.R.S., Metabolism and Radiation Research Laboratory.

² Appreciation is expressed to B. R. Lewton for technical assistance during this study.

³ Reference to a company or product name does not imply approval or recommendation of the product by the U.S. Department of Agriculture to the exclusion of others that may be suitable.