HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Fowler, A. K. Articles by Reed, C. D. Search for Related Content PubMed PubMed Citation Articles by Fowler, A. K. Articles by Reed, C. D.

Estrogen Analysis of Neonatal Bovine Urine Using Gas-Liquid Chromatography^{1, 2, 3,}

University of New Hampshire, Durham 03824

Abstract

ESTRADIOL-17 and estrone were identified by gas-liquid chromatography as the major estrogen metabolites in the phenolic extract of neonatal bovine urine. Estrogen identification was made by the comparison of retention times to reference standards as trimethylsilyl ether derivatives and by peak area comparison of chromatograms obtained using 4 different stationary liquid phases (NGS, SE-30, QF-1 and XE-60: SE-30). A compound having a similar retention time as authentic estradiol-17ß was found on all columns; however, inconsistencies in the peak area of chromatograms made identification tentative. The highest mean urinary levels of estradiol-17 and estrone were recovered at one day of age. These levels then decline exponentially (P<.O5) with only three out of six calves having detectable levels at 16 days of age. Significant differences among calves within sex and between sexes (P<.OS) were noted in the concentration (µg/liter urine) of estrone, but not estradiol-17. These differences, however, were not significant (P>.05) when estrone levels were expressed as µg/mg urinary creatinine.

¹ Published with the approval of the Director of the New Hampshire Agricultural Experiment Station as Scientific Contribution No. 501.

² We wish to acknowledge the kind assistance of Willard Urban for the statistical interpretation of these data.

³ Department of Animal Science.

⁴ Present address: Etiology Group, National Cancer Institute, Bethesda, Maryland 20014.