J. Anim Sci.
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J. Anim Sci. 1972. 35:580-584.
© 1972 American Society of Animal Science
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Interaction of Extender Composition and Incubation Period on Cold Shock Susceptibility of Boar Spermatozoa

V. G. Pursel, L. A. Johnson and L. L. Schulman

U.S. Department of Agriculture,1 Beltsville, Maryland 20705

Abstract

Four trials were conducted to determine the effects of extender composition on the development of cold shock resistance in boar spermatozoa. Acrosome morphology and sperm motility were the criteria used to assess cold shock damage. Spermatozoa developed Cold shock resistance during in vitro incubation at 30 C in all four trials. Extender pH had a marked effect on the development of cold shock resistance. When spermatozoa were cold shocked after 2-hr, incubation, sperm motility in the extenders with pH 7.3, 8.0 and 8.3 was significantly (P<.01) higher than in the extenders with pH 5.9 and 6.6. However, sperm acrosomes were significantly (P<.01) more resistant to cold shock after 7-hr, incubation in extenders with pH 5.9, 6.6 and 7.3 than in extenders with pH 8.0 and 8.3. Sperm acrosomes were significantly (P<.01) more resistant to cold shock after 5-hr, incubation in the lactose, sucrose and raffinose containing extenders than in the glucose and fructose containing extenders. Varying the level of Tris in the extender from 0.082M to 0.246M did not significantly affect the cold shock resistance developed during 1- or 5-hr, incubation. Varying the level of egg yolk in the Tris-lactose extender from 0 to 30% had no significant effect on sperm motility after cold shock at 1- or 5-hr, incubation. Sperm acrosomes were more cold shock resistant after 5-hr, incubation in 0% egg yolk extender than in 30% egg yolk extender (P<.01) and 20% egg yolk extender (P<.05).

Footnotes

1 Animal Science Research Division, A.R.S.






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