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Lysine Availability of Opaque-2 Corn for Rats¹

Purdue University, Lafayette, Indiana 47907

Abstract

Three experiments were conducted to estimate the lysine availability of normal corn and two varieties of opaque-2 corn for the the weanling rat using the growth assay method. A wheat gluten basal diet, deficient in lysine but otherwise nutritionally adequate, was used with graded levels of crystalline lysine for the reference curve and graded levels of lysine supplied by the normal corn or two varieties of opaque-2 corn (V1 and V2) for the response curves. Using regression analysis (weight gain vs. lysine consumed) and applying a slope ratio technique, the average lysine availability estimates for normal, opaque-2 corn V1 and opaque-2 corn V2, were 70%, 80% and 89%, respectively.

¹ Department of Animal Sciences Journal Paper No. 4628, Purdue University Agricultural Experiment Station.

² Present address: Indiana Farm Bureau Cooperative Association, Inc., Central Laboratory, 47 South Pennsylvania Street, Indianapolis, Indiana 46204.

³ Department of Animal Sciences.

⁴ Department of Biochemistry.