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Effects of Synthetic Luteinizing Hormone-Releasing Hormone/Follicle Stimulating Hormone-Releasing Hormone (LH-RH/FSH-RH) on Serum LH, Serum FSH and Ovulation in Anestrous Ewes^{1, 2, 3,}

Washington State University, ^,4 Pullman, Washington 99163 , VA Hospital, Tulane Medical School, ^,5 New Orleans, Louisiana 70140 and University of California,^,6 San Francisco Medical Center, San Francisco, California 94122

Abstract

Five anestrous ewes were each treated intramuscularly with a total dose of 400 µg synthetic LH-RH/FSH-RH, given in two equal doses at a 4-hr, interval. Five control ewes were identically treated with carrier medium, acidified saline. Laparotomies were conducted 2 days before and 3 days after the treatment and presence of corpora lutea was used as an indication of ovulation. Double antibody radioimmunoassays were used to quantitate both serum LH and FSH concentrations before and after treatment. The mean peak concentration of serum LH and FSH of the ten treatments in the five LH-RH/FSH-RH treated ewes was 39.1±6.8 and 246±27 ng/ml, respectively. The mean times of occurrence of the LH and FSH peaks after intramuscular treatment with synthetic LH-RH/FSH-RH were 96±15 and 101±11 min., respectively. There was considerable variation in magnitude of LH and FSH release as well as the shape of the curves after treatment with LH-RH/FSH-RH between ewes and within the same ewe after two identical treatments. Ovulation occurred in three of the LH-RH/FSH-RH treated ewes and one of the saline treated control ewes, indicating that these ewes were all close to spontaneous ovulation probably due to the onset of the breeding season. There seemed to be little correlation between magnitude of serum LH or FSH concentrations and ovulation in the ewes treated with synthetic LH-RH/FSH-RH.

¹ Scientific Paper 3777 College of Agriculture Research Center, Washington State University. Project 1695.

² The authors would like to thank Dr. H. Matsuo for providing us with a sample of synthetic LH-RH/FSH-RH; Dr. Leo Reichert for the LH used for iodination; Dr. Harold Papkoff for the FSH used for iodination; Dr. William White for antiovine LH serum; and the Endocrine Study Section NIH for the LH and FSH standards.

³ This study was supported in part by USPHS Grant from the National Institute of Child Health and Human Development AM 06192 (to J. J. R.), AM 09094 (to A. A.), AM 07467 (to A. V. S.), and University of California #13 Moffitt fund and MSC #21 (to C. L. K.).

⁴ Department of Animal Sciences.

⁵ Endocrine and Polypepide Laboratories, Department of Medicine.

⁶ Department of Physiology.

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