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Establishment of a Rumen Pouch in Sheep and Design of a Pouch Perfusion System^{1, 2,}

University of Massachusetts, Amherst 01002

Abstract

The surgical procedure described in this paper enables the formation of rumen pouches from the dorsal sac of the rumen. The rumen pouch provides a method for the study of transport across rumen epithelial tissue under more nearly physiological conditions than exist in most other procedures. Alteration of results by rumen fatty acid production, anesthesia, or severe stress at the time of study is eliminated. Also, the same animal may be used under similar conditions for several trials.

The perfusate circulatory systems described in this paper prevent stagnation or development of a concentration gradient in the test solution within the pouch. These systems insure sufficient volume of test solution for sampling throughout the study. They also provide an effective means to change test solutions during a particular trial. Then rumen pouch in conjunction with the perfusate circulatory system provides an excellent method to study transport of substances across the rumen epithelium in vivo.

¹ The assistance of Dr. R. T. Duby with the surgery is appreciated.

² The financial aid to the senior author by the Ralston Purina Company of St. Louis is gratefully acknowledged.

³ Present address: Department of Animal Sciences, University of Vermont, Burlington, 05401.