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In Vitro Progesterone Synthesis by Corpora Lutea Induced in Prepuberal Cattle^{1, 2,}

Cornell University, Ithaca, New York 14850

Abstract

Superovulation was induced in prepuberal calves using PMS and LH. The induced CL were collected at 5, 10, 15 or 20 days after the LH injection. Initial progesterone concentrations were greatest at 5 days, but remained high even to day 20. Upon incubation CL obtained after 5, 10 and 15 days synthesized more progesterone (P<.05) than 20-day CL in the control medium and in media containing LH or NADPH. The overall stimulation of progresterone synthesis by LH was 27% and by NADPH was 60% when compared to control incubations. All incubated CL utilized glucose, but in no case was glycolysis stimulated by LH. NADPH stimulated glycolysis in 5-, 10- and 15-day CL incubations. It did not stimulate glycolysis at 20 days even though these incubations could still be stimulated to synthesize progesterone by NADPH. The accumulation of lactate in vitro was not affected by LH or by NADPH. Asynchronous regression of CL was observed both within and among animals at 20 days, a fact which may in part be due to different CL ages resulting from variable ovulation times in superovulated animals. Of major interest in these studies was the fact that an abundance of functional luteal tissue was formed in prepuberal calves following superovulation. This tissue responded to LH and NADPH in vitro by increasing progesterone synthesis.

¹ Department of Animal Science.

² Suggestions concerning the manuscript by Dr. W. Hansel and the technical assistance of Miss J. Wiebold and Mr. R. Cole are appreciated. The hormones used were generously donated by Armour-Baldwin Laboratories (PLH), Ayerst Laboratories, Inc. (Equinex), and the Endocrinology Study Section, NIH (NIH-LH-B5). This work was partially supported by NIH Grant HD 03471.

³ Present address: Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545.

⁴ Present address: Department of Physiology and Biophysics, Colorado State University, Ft. Collins 80521.