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Bovine Serum Lipids. III. Qualitative Changes from the Isolated Perfused Bovine Liver¹

University of Arizona, Tucson 85721

Abstract

The livers from eight steers that had been fed diets containing four different levels of added animal fat were isolated and perfused with their own defibrinated blood. Blood samples were collected before, during and after each 2-hr, perfusion period. Sera were collected by centrifugation and the sera lipids isolated, fractionated and prepared for gas-liquid chromatography by standard analytical procedures. Analysis of variance revealed that the control ration accounted for a significant (P<.05) decrease in linoleic acid percentage in the phospholipid fraction. Between initial perfusion time and 30 min. of perfusion, independent of diet, there was a significant (P< .05) increase in stearic acid and a significant (P<.05) decrease in oleic acid in the free fatty acid fraction. Analysis of the unfractionated sera lipids indicated a slight but significant increase in palmitoleic acid, a relatively minor acid, over time of perfusion. Diet or time of perfusion effects were not significant for any major fatty acids in the unfractionated sera.

¹ Arizona Agricultural Experiment Station Technical Paper 1848.

² Present address: Food Science Department, University of Missouri, Columbia 65201.