J. Anim Sci.
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J. Anim Sci. 1972. 34:660-671.
© 1972 American Society of Animal Science
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A Plasma Reference Index for Predicting Limiting Amino Acids of Sheep and Rats1

E. L. Potter2, D. B. Purser3 and W. G. Bergen4, 5,

Michigan State Univerity, East Lansing 48823

Abstract

Experiments were conducted to assess the appropriateness of two procedures, based on plasma amino acid data, for the determination of limiting amino acids. The first procedure, the PAA Index did not identify the limiting amino acid of various dietary protens when fed to rats or when infused into the duodenum of sheep. The second procedure utilized a plasma amino acid reference pattern which was derived by feeding rats, bottle feeding lambs and goats or duodenally infusing sheep with high quality proteins. A plasma amino acid reference index (PAARI) was then calculated by expressing the plasma amino acid levels resulting from test proteins as a percent of the reference pattern. The lowest percent value indicated the limiting amino acid. For the species tested, the PAARI procedure correctly identified the supposed limiting amino acid of the various protein sources tested. The reference pattern derived here for rats and sheep also correctly identified the limiting amino acids of various proteins from some of the plasma amino acid data previously published. The PAAR-Index requires the establishment of an appropriate plasma amino acid reference pattern. Presumably such reference patterns would have to be established for each specie uner study as well as for various physiological states of the animal.

Footnotes

1 Published with the approval of the Director of the Michigan Agricultural Experiment Station as Journal Article No. 5605. Supported in part by the Biomedical Sciences Support Grant, 5-505-FRO7049, U. S. Public Health Service.

2 Present address: Department of Animal Science, Ohio Agricultural Research and Development Center, Wooster.

3 Present address: C.S.I.R.O., P.O. Private Bag, Wembly, W. Australia.

4 Ruminant Nutrition Laboratory, Department of Animal Husbandry.

5 The help of Dr. W. T. Magee during the statistical evaluation is gratefully acknowledged.






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Copyright © 1972 by the American Society of Animal Science.