J. Anim Sci.
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J. Anim Sci. 1972. 34:563-568.
© 1972 American Society of Animal Science
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A Method for the Separation of Fiber Types from Skeletal Muscle1

M. Mowafy, L. L. Kastenschmidt and R. G. Cassens

University of Wisconsin,3 Madison 53706

Abstract

A technique for separation of red and white fibers from skeletal muscle was developed; the procedure is based on enzyme digestion of connective tissue and subsequent separation of fiber types in a sucrose density gradient. A light fixation of the muscle in glutaraldehyde was prerequisite, as the technique was successful with the present technique. This liminary trials showed that modification of the technique is necessary if it is to be applied to other muscles or species.

Footnotes

1 Research supported by the College of Agricultural and Life Sciences, University of Wisconsin, Madison, by a grant from the American Meat Institute Foundation and by Public Health Services Grant No. FD-00107-13. Muscle Biology Laboratory Manuscript No. 20.

3 Department of Meat and Animal Science.






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Copyright © 1972 by the American Society of Animal Science.