HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Brinsfield, T. H. Articles by Reynolds, P. J. Search for Related Content PubMed Articles by Brinsfield, T. H. Articles by Reynolds, P. J.

Cytoplasmic Degeneration in the Wool Follicle Bulb Following Administration of Cyclophosphamide¹

U.S. Department of Agriculture, ² Beltsville, Maryland 20705

Abstract

The effect of CPA on the wool follicle bulb was studied in each of five ewes at 0, 3.5, 24, 48 and 72 hr. after treatment with either 25 or 75 mg of CPA per kilogram of live weight. Cytoplasmic inclusions first appeared in the germinative cells of the wool follicle bulb at 24 hr. after CPA treatment and continued to be present, but in smaller numbers, at 48 and 72 hr. but were no longer present by 168 hr. after treatment. Frozen sections of skin incubated for the demonstration of acid phosphatase showed that these cytoplasmic inclusions contained acid phosphatase. The morphological and histochemical findings suggest that these inclusions are lysosomes. Results suggest that CPA may act as a defleecing compound by temporarily altering cell function and, consequently, inducing cytoplasmic degeneration in some of the germinative cells of the wool follicle bulb, thus interfering with normal wool growth.

¹ The authors thank Dr. H. Leo Dickison. Bristol Laboratories, Syracuse, New York, for providing the cyclophosphamide. The technical assistance of Pamela Myerovitz and R. J. Schied is greatly appreciated.

² Agricultural Research Service. A.R.S.