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Porcine Plasma Growth Hormone Levels: Radioimmunoassay Technique and its Application¹

Purdue University, Lafayette, Indiana 47907

Abstract

A sensitive double antibody radioimmunoassay for porcine growth hormone is described. The assay was sensitive to 0.2 ng and was shown to be specific for only growth hormone by measuring the antigen activity of pituitary proteins separated by polyacrylamide disc gel electrophoresis. Plasma growth hormone levels were not significantly different between stress susceptible and stress resistant pigs. Observation of arterial and venous growth hormone levels indicated that arterial levels were significantly higher (P<.025) than venous levels and that stress susceptible swine may have a higher rate of tissue utilization and/or metabolism of circulating growth hormone. Slowly alternating environmental temperature (21 C to 32 C to 21 C once per 24 hr.) increased plasma growth hormone levels; however, more rapid and more extreme alternation of temperature (12 C to 32 C to 12 C once per 4 hr.) did not have a significant effect.

¹ Journal Paper No. 4431 of the Purdue Agricultural Experiment Station, Department of Animal Sciences.

² Present Address: Muscle Biology Laboratory, University of Wisconsin, Madison.

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