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Effect of Feeding Corn Inoculated with Diplodia Zeae in Laboratory Animals^{1, 2,}

The Ohio State University, Columbus, Ohio 43210 and The Ohio Agricultural Research and Development Center, Wooster 44691

Abstract

DIGESTIBILITY of protein and its nutritive value as well as energy content were reduced by infection of corn with Diplodia ear rot (Diplodia zeae) when measured by growth of rats (Mitchell and Beadles, 1940). Adelaar (1958) reported that infection of maize cobs and stalks with this fungus was one of the causes of paralysis in cattle. Teague (1966) reported that Diplodia zeae was one of the molds isolated from the corn crop grown in areas of Ohio, Indiana, Michigan and Ontario during 1965.

The objective of this study was to determine the effect of feeding corn inoculated with cultures of Diplodia zeae to rats, mice, hamsters and guinea pigs.

A completely randomized design was used in this study. This together with the distribution of animals by treatment is presented in table 1. The animals, weaned at 3 weeks of age, were obtained from laboratory animal facilities of The Ohio State University and kept in separate 33 cm long, 20 cm wide and 20 cm high cages.

¹ Approved as Journal Article No. 34-70 by the Associate Director of The Ohio Agricultural Research and Development Center, Wooster.

² The authors acknowledge the cooperation and assistance of Richard Ritter and Getz Read of The Ohio Agricultural Research and Development Center and Harold Delong of The Ohio State University.

³ Graduate Assistant, Department of Animal Science, Columbus; Professor, Department of Animal Science, Columbus; Professor, Department of Plant Pathology, Wooster; and Associate Professor, Department of Animal Science, Columbus.