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U.S. Department of Agriculture, ,2 Albany, California 94710 and University of Nebraska, Lincoln 68503
Abstract
A convenient enzymatic procedure has been developed which is designed to predict in vivo dry matter digestibility of forages and crop residues. The procedure involves digestion of the forage with a crude cellulose enzyme followed by a protease digestion. The procedure does not require the close proximity of a ruminant animal.
Enzymatic solubilization of 29 forages was highly correlated (r=.900) with in vivo dry matter digestibility. Correlations were higher within species than among species. The procedure appears to predict dry matter digestibility with sufficient precision to be of use to laboratories with no access to donor ruminant animals.
1 The technical assistance of Miss Erlinda Reyes, Miss Margaret Connor and Mrs. Ann Gramps is gratefully acknowledged. Financial support for Miss Reyes was furnished by Oregon State University, Corvallis, Oregon. Thanks are due to M. D. Miller of the University of California at Davis, R. F. Barnes of Purdue University, Pacific Vegetable Oil Corporation, Delta Dehydrating Co., J. G. Boswell and Co., and the Alfalfa Dehydrators Association for samples of materials examined.
2 Agricultural Research Service, Western Regional Research Laboratory, Albany, California. Reference to a company or product name does not imply approval or recommendation of the product by the U.S. Department of Agriculture to the exclusion of others that may be suitable.
3 Approved by the Director as Paper No. 2910, Journal Series, Nebraska Agricultural Experiment Station, Lincoln.
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