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The Sulfobromophthalein (BSP) Liver Function Test for Sheep¹

University of Kentucky, Lexington 40506

Abstract

Studies were conducted to adapt the sulfobromophthalein (BSP) liver function test to sheep. The dosage of BSP required to accurately measure blood clearance of this dye in sheep was found to be 15 to 20 mg per kilogram of body weight, which is about three times the dosage used in clinical medicine. From 82 to 83% of the injected dye was excreted in the bile within 60 min. after injection while less than 1.0% appeared in the urine. Chromatography revealed three fractions of BSP in the excreted bile. One of these fractions chromatographed as free BSP, whereas two of the fractions appeared to be conjugated with ninhydrin-positive compounds. Direct liver injury by carbon tetrachloride poisoning greatly reduced the ability of the ovine liver to excrete BSP. These findings suggest that the BSP liver function test for sheep is useful if the dosage is increased sufficiently to compensate for the rapid clearance by this species.

¹ The investigation reported in this paper (No. 70-5-118) is in connection with a project of the Kentucky Agricultural Experiment Station and is published with the approval of the Director.