J. Anim Sci.
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J. Anim Sci. 1971. 32:711-715.
© 1971 American Society of Animal Science
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Hydrolysis of 15N-Biuret by In Vitro Rumen Fermentation and Ruminal Biuretase

W. R. Bauriedel, L. F. Craig, J. C. Ramsey and E. O. Camehl

The Dow Chemical Company,1 Midland, Michigan 48640

Abstract

Conclusions: The use of 15N-labeled biuret has permitted the direct demonstration that ammonia, the fundamental nitrogen source for rumen microorganisms, is a product of the fermentation of biuret by rumen microorganisms. The extent of biuret hydrolysis in our in vitro system did not appear to be greatly affected by the amount of energy-providing substrate present, but the incorporation of the 15NH3 produced into microbial protein was promoted by the addition of carbohydrate substrate. Cell-free hydrolysis of 14C-15N-biuret by a crude ruminal biuretase preparation yielding 14CO2 and 15NH3 has been demonstrated.

Footnotes

1 Agricultural Department.






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