J. Anim Sci.
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J. Anim Sci. 1971. 32:287-291.
© 1971 American Society of Animal Science
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Effects of Benzimidazoles as Inhibitors of the Metabolism of Washed Porcine Spermatozoa1

W. L. Witters and C. W. Foley

Ohio University,2 Athens 45701 and University of Georgia,,3 Athens 30601

Abstract

Three side-chain substituted benzimidazoles, DMB, EMB and HMB were incubated with washed boar sperm. Of the three, only HMB treatments significantly (P<.01) increased the pyruvic acid production by sperm. Different rates of inhibition were noticed for the benzimidazoles on lactic acid production with HMB inhibiting the most and DMB the least. The pH of EMB and DMB treated samples increased significantly (P<.01) more than controls. HMB samples showed no significant pH change but were lower in pH than samples containing the other inhibitors. HMB and the 300 mcg levels of EMB and DMB significantly lowered (P<.01) motility of sperm over 2 hours. Samples treated with all three inhibitors had significantly (P<.01) higher oxygen consumption after 30 min. of incubation but only EMB and DMB treated samples had significantly higher oxygen for 2 hr. of incubation. The effects on oxygen consumption are similar to those of oxidative uncoupling but more tests should be run on ATP level to verify this. The metabolic mode of action of benzimidazoles is unknown at present. Studies with polio and influenza virus have shown that RNA synthesis is blocked but no comparable investigation has been made with spermatozoa.

Footnotes

1 Journal Paper No. 4069, Purdue University Agricultural Experiment Station, Lafayette, Indiana 47907. A contribution from the Department of Animal Sciences.

This investigation was supported in part by NDEA Title IV Grant, 7654.

2 Department of Zoology.

3 Department of Physiology and Pharmacology.






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