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Excretion of 4-¹⁴C Corticosteroids in Urine by an Ovariectomized Bovine¹

Purdue University, Lafayette, Indiana 47907

Abstract

A 300-kg ovariectomized heifer was used to determine the percentages of injected radioactivity from corticosterone and cortisol that appear in the urine of the bovine. This was done by intravenously injecting 4-¹⁴C-corticosterone (4.96 µc) and 4-¹⁴C-cortisol (4.28 µc) with a 19-day interval between injections and measuring the urinary radioactivity following the injections. Complete urine collections were taken via catheter.Radioactivity was detected in the urine 10 min. after injection of the ¹⁴C-cortiscosterone (at the first sampling period). By collecting samples at 2-min. intervals after injection of ¹⁴C-cortisol, radioactivity was detected at 4 min. after injection. More than one-third of the total urinary radioactivity was recovered during the first 12 hr. for both corticosteroids. The accumulated percentages of the injected doses of ¹⁴C-corticosterone and ¹⁴C-cortisol were 20.2% and 29.0%, respectively, by 72 hr. after injection. Urine radioactivity returned to background levels by 72 hours.

¹ Paper No. 4051, Purdue University Agricultural Experiment Station. A contribution from the Department of Animal Sciences.

² Fellow, National Aeronautical and Space Agency; Grant No. 2779–82.