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Excretion of ¹⁴C in Urine of the Domestic Sow after Injection of Radioactive Estradiol-17ß, Estrone, Corticosterone and Cortisol¹

Purdue University, Lafayette, Indiana

Abstract

Two yearling sows, one intact and one ovariectomized, were used to determine the rate of excretion in urine of ¹⁴C following intravenous injection of ¹⁴C-steroids. Estradiol-17 ß-4-¹⁴C (2.26 µc) and estrone-4-¹⁴C (2.12 µc) were singly injected into the intact sow during two estrous periods. The interval between injections was 6 weeks. Five months later corticosterone -4-¹⁴C (4.74 µc) and cortisol-4-¹⁴C (4.41 µc) were singly injected on day 19 of two successive estrous cycles. Only cortisol-4-¹⁴C (4.25 µc) was injected into the ovariectomized sow.

Radioactivity appeared in the urine of both sows within the first 10 min. after injection of each steroid. Recoveries of ¹⁴C in urine of the intact sow 144 to 168 hr. after injection were 89, 86, 80 and 79%, respectively, for estradiol-17ß estrone, corticosterone and cortisol. The ovariectomized sow excreted in urine 97% of the injected ¹⁴C-cortisol in 120 hours. Twelve hours after injection of the intact sow 64% of the ¹⁴C from cortisol and 33% of the ¹⁴C from corticosterone had been recovered in the urine. Excretion of ¹⁴C from estrone was considerably greater for the first 10 min. as compared to estradiol-17 ß but total recovery of ¹⁴C in urine for the latter compound by 12 hr. was greater (79% vs. 70%).

¹ Journal Paper No. 4035, Purdue University Agricultural Experiment Station, Lafayette, Indiana 47907. A contribution from the Department of Animal Sciences.

² Present address: Department of Dairy, Michigan State University, East Lansing 48823.

³ A Fellow, National Aeronautical Space Agency, Grant No. 2779–82.

⁴ A Fellow, Purdue Research Foundation, Grant No. 5076.