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Meat and Bone Meals as Sources of Amino Acids for the Growing Rat: Use of a Reference Diet to Predict Amino Acid Adequacy by Levels of Plasma Free Amino Acids^{1, 2, 3,}

University of Minnesota, St. Paul

Abstract

LEVELS of free amino acids in the plasma should be excellent response criteria for predicting the limiting amino acids and availability of amino acids from different proteins. The use of concentrations of free amino acids in the plasma takes into account digestibility and availability which are not known when amino acid adequacy is predicted from chemical analysis of protein. A suitable reference diet will contain neither excesses nor inadequate amounts of amino acids and will support maximal performance of the test animal to establish baseline or reference levels for each of the amino acids in the plasma.

Two experiments were conducted to determine dietary deficiencies and excesses of amino acids in meat and bone meals from different origins by comparing the plasma free amino acid levels of rats fed the meat and bone meal diets with those of rats fed caseinamino acid reference diets. The effect of reducing the levels of certain amino acids in the reference diet on predicting dietary deficiencies and excesses of amino acids in the meat and bone meals was determined.

¹ Paper 7197, Scientific Journal Series of the Minnesota Agricultural Experiment Station.

² Data from a thesis submitted to the Graduate School, University of Minnesota, by the senior author in partial fulfillment of the requirements for the degree of Doctor of Philosophy.

³ Supported in part by a grant-in-aid from the Fats and Proteins Research Foundation, Des Plaines, Illinois.

⁴ NIH Trainee in Nutrition; supported by NIH Training Grant GMO 1041 from the National Institute of General Medical Sciences.