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Enzyme Activities of Ruminant Digestive Tract Epithelial Tissue^1,2,

Oregon State University, Corvallis

Abstract

Steers were fed rations containing either cottonseed meal or urea as the principal supplementary nitrogen source. When they were slaughtered, epithelial tissues from various parts of the digestive tract were removed for comparison of the enzymatic activity of these tissues with that of the liver. Comparison of the activity of several enzymes in the liver and rumen epithelium involved in nitrogen metabolism was also made. Lactic dehydrogenase, -hydroxybutyric dehydrogenase, isocitrate dehydrogenase, glutamic dehydrogenase and aryl sulfatase levels were highest in the liver, whereas malic dehydrogenase, the malic enzyme, D-glyceraldehyde-3-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase were, respectively, highest in epithelial tissue from the reticulum, rumen, omasum, abomasum and small intestine (duodenum). No malic enzyme was detected in the liver, but it. was present in the heart, kidney, spleen, lungs, tongue, muscle and adipose tissue. Except for ornithine transcarbamylase and glutamic-pyruvic transaminase in the liver and tyrosine transaminase and glutamic-oxalacetic transaminase in rumen epithelium, there was a slight increase in activity of the enzymes studied (ornithine transaminase, ornithine transcarbamylase, arginase, tyrosine transaminase, glutamic-oxalacetic transaminase, glutamic-pyruvic transaminase, tryptophan transaminase, phenylalanine transaminase, and glutamic dehydrogenase) in the liver and rumen epithelium of animals fed diets containing urea.

¹ Oregon State Agricultural Experiment Station Technical Paper No. 2766.

² The authors wish to thank Margaret Olson and Susan Carnegie for their technical assistance, and Dr. W. H. Kennick for assistance in collecting the tissue samples.

³ Department of Agricultural Chemistry.

⁴ Department of Animal Science.