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Effect of Grain Processing on in vitro Gas Production¹

University of Arizona, Tucson

Abstract

In Vitro rumen fermentation systems have been successfully used to evaluate cellulose digestion in roughages and the findings have been effectively related to in vivo utilization (Johnson, 1963). Only limited use has been made of in vitro systems to evaluate grain digestion or utilization by rumen microorganisms. In vitro fermentation methods have been used to study utilization of starch by rumen microorganisms (Salisbury, Hoeffer and Luecke, 1961; Moore, Johnson and Dehority, 1962; Loper, Little and Mitchell, 1966). The methods as used by them required starch measurements following the incubation period. Due to the difficulty in determining starch, the methods do not lend themselves to routine use. Several workers have used gas production as a measure of fermentation with forage studies. The problems of gas production as a measurement in in vitro systems have been discussed by Johnson (1963).

In evaluating various methods of grain processing, the need arose for a rapid, simple in vitro technique for evaluating the influence of various processing factors and predicting their values to the intact animal.

¹ Arizona Agricultural Experiment Station Technical Paper No. 1515.

² This paper is part of the dissertation submitted by the senior author to the Graduate College, University of Arizona, Tucson, in partial fulfillment of requirements for the Ph.D. Degree.

³ Present address: Smith Kline & French Laboratories, West Chester, Pennsylvania.

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