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Continuous In Vitro Culture System for Ruminant Research I. Design^1,2,

University of Illinois, Urbana⁴

Abstract

A continuous in vitro culture system for ruminant research was made using six @plexiglass fermentation chambers (each equipped with magnetically-driven stirrers), a plexiglass typater bath equipped with an immersion heater, a peristaltic pump, six rubber basketball bladders, six 4-liter polyethylene bottles and a one-quarter horse power electric motor with a gear reduction box, A buffer solution was continuously infused into the fermentation chambers by a peristaltic pump, and the liquid and gas residues were collected in polyethylene bottles and rubber bladders, respectively. The fermentation chambers were in a heated water bath for constant temperature regulation. The electric motor powered two submerged, parallel plexiglass shafts, each of which provided agitation for three fermentors through magnetically-driven stirrers inside each fermentation chamber.

¹ Data taken from thesis submitted by the senior author to the Graduate College, University of Illinois, in partial fulfillment of the requirements for the Ph.D. degree.

² Supported in part by federal funds (Hatch 20–346 and 20–348) and by a grant-in-aid from Dow Chemical Company, Midland, Michigan.

³ Present address: Department of Meat and Animal Science, University of Wisconsin, Madison.

⁴ Animal Science Department.