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Effects of Cold Shock, Dilution, Glycerol and Dimethyl Sulfoxide on Cation Concentrations in Porcine Spermatozoa¹

Purdue University, Lafayette, Indiana

Abstract

Boar sperm were cold shocked by cooling from 26° to 16°, 6° or –4 C, with or without dilution (1:4) in modified IVT diluent. Sperm gained significant (P.01) amounts of sodium and zinc and lost potassium at 6° and –4°. Cold shocked sperm lost magnesium in diluted semen but not in undiluted semen. Such changes in cation content of boar sperm also took place due to cold shock at 0 C in diluted semen. Dimethyl sulfoxide (7.5%) caused sperm to lose sodium, potassium and magnesium and gain zinc. Glycerol (7.5%) caused loss of potassium and magnesium and uptake of zinc. Neither glycerol nor DMSO alleviated the effects of cold shock on cation content of boar sperm.

¹ Journal Paper No. 3609, Purdue University Agricultural Experiment Station, Lafayette, Indiana. A contribution from the Department of Animal Sciences.

² Present address: Department of Biology, University of Alabama, University, Alabama 35486.

³ Present address: Department of Physiology, University of Georgia, Athens, Georgia 30601.