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University of Wisconsin, Madison3
Abstract
Sarcoplasmic, myofibrillar and total muscle protein were extracted from biopsies obtained from the longissimus of both steers and heifers at various stages of growth. Total muscle protein decreased in amount as the animals deposited intramuscular lipid. The relative amount of sarcoplasmic proteins increased from 12 months of age to 16 months and then decreased until termination of the experiment at 22 months of age. Stromal protein, determined by difference, did not change in a regular pattern during the time period studied. Myofibrillar protein changed inversely to the sarcoplasmic protein. Myoglobin and residual hemoglobin, as measured by the optical density of the sarcoplasmic protein fraction, paralleled changes in the sarcoplasmic protein concentration.
1 This investigation was supported in part by U.S.D.A. contract No. 12-14-100-8295 (61), from the Human Nutrition Research Division of the Agricultural Research Service.
2 Present address: Department of Animal Science, Texas A&M University, College Station.
3 Published with the approval of the Director of the Wisconsin Agricultural Experiment Station. Department of Meat and Animal Science Manuscript No. 531.
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