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Method for Isolating the Rumen of Sheep In Situ

University of Alberta, Edmonton

Abstract

Procedures for the surgical preparation of omasal, rumen and esophageal fistulas and the construction of the required cannulas were developed. Four mature Suffolk ewes and eight yearling lambs were fitted with the three fistulas and the fistulas utilized for short-term isolation of the reticulo-rumen. The reticulo-rumen isolation technique prevented fluids from leaking into or out of the rumen via the esophagus or omasal orifices. No obvious adverse effects of the reticular-rumen isolation procedure on the health of the animals were observed. Saliva secretion rates were increased immediately following the reticulo-rumen isolation and returned to control values approximately 90 min. after isolation. Saliva secretion rates, measured 85 to 90 min. after reticulo-rumen isolation, tended to be higher 17 hr. postfeeding, than 3 hr. postfeeding. Decreasing the pH of rumen ingestion did not significantly alter saliva secretion rates.

¹ Present address: Food and Drug Directorate Research Laboratories, Tunney's Pasture, Ottawa 3, Canada.

² Present address: Department of Animal Husbandry, University of California. Davis.

³ Department of Animal Science.