J. Anim Sci.
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J. Anim Sci. 1969. 29:51-56.
© 1969 American Society of Animal Science
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Comparative Blood Ammonia Procedures in Ruminants1

R. L. Preston2

University of Missouri,,3 Columbia

Abstract

Three procedures were compared which are reported to minimize artifactual ammonia formation when whole blood is analyzed for ammonia-N. Using jugular blood obtained from sheep, the borate buffer procedure of Reinhold and Chung resulted in the least formation of artifactual ammonia. All three procedures gave parallel results indicating that valid relative comparisons can be made with all three procedures. For routine laboratory determinations, the borate buffer procedure is the easiest to employ if the blood samples can be analyzed immediately after withdrawal.

Footnotes

1 Contribution from the Missouri Agricultural Experiment Station. Journal Series No. 5580. Approved by the Director.

2 Assistance rendered by L. Kerns is gratefully acknowledged.

3 Department of Animal Husbandry.






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Copyright © 1969 by the American Society of Animal Science.