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University of Wisconsin, Madison
Abstract
Studies were conducted to determine whether a difference existed between the calcium accumulating ability of sarcoplasmic reticulum fragments from muscles that ultimately became normal or PSE. The sarcoplasmic reticulum fragments from normal muscle contained significantly more contaminating, salt-soluble proteins than PSE preparations. The calcium accumulating ability of purified sarcoplasmic reticulum fractions from normal muscle was higher (P<.01) than that of similar preparations from PSE muscle. There was no evidence obtained that indicated that the calcium accumulating activity declined between biopsy and death in the crude sarcoplasmic reticulum fractions. These results suggested that the differences observed between normal and PSE preparations were inherent and may not reflect a more rapid postmortem change in muscles that became PSE.
1 Published with the approval of the Director of the Wisconsin Agricultural Experiment Station, Department of Meat and Animal Science, Manuscript No. 544. The senior author expresses appreciation for a National Science Foundation Fellowship. This research was supported in part by Public Health Service Research Grant UI-00266-09 from the National Center for Urban and Industrial Health, in part by the Armour Food Research Laboratories and in part by the American Meat Institute Foundation.
2 Present address: Retina Foundation, Department of Muscle Research, Boston, Massachusetts.
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