J. Anim Sci.
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J. Anim Sci. 1969. 28:523-530.
© 1969 American Society of Animal Science
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Behavior of Swine Leucocytes during in vitro Culture1

A. F. McFee and M. W. Banner2

Agricultural Research Laboratory of the University of Tennessee,3, Oak Ridge

Abstract

The response of swine leucocytes to in vitro stimulation with phytohemagglutinin was evaluated over a 96-hr. culture period in order to establish their basic behavior and to define quantitative measures which could be used to appraise the effects of various insults on these cells. Little change was noted for the first 24 hr. thereafter, cell number, cell viability and pH declined steadily, except that cell losses were compensated for by division after 72 hours. Cells labeled with tritiated thymidine appeared in significant numbers at 24 hr. and increased to a peak of 30% of the population at 72 to 84 hours. Mitotic figures were noted at 36 hr. and reached their maximum at 84 hours. Only cells of the mononuclear series showed evidence of being stimulated. The mean volume of cell nuclei, as measured by an electronic counter, increased by 38% during the 96-hr. period; this procedure seems to hold promise as a method for quantitating the ability of leucocytes to respond to growth-promoting stimulation.

Footnotes

1 Published with the permission of the Dean of the University of Tennessee Agricultural Experiment Station, Knoxville.

2 The authors gratefully acknowledge the technical assistance of Mary N. Sherrill and Fannie H. Cross.

3 Operated by the Tennessee Agricultural Experiment Station for the U. S. Atomic Energy Commission under Contract No. AT-40-1-GEN-242.






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Copyright © 1969 by the American Society of Animal Science.