J. Anim Sci.
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J. Anim Sci. 1969. 28:369-374.
© 1969 American Society of Animal Science
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The Male Rabbit. III. Determination of Daily Sperm Production by Means of Testicular Homogenates1

R. P. Amann and J. T. Lambiase, Jr.

The Pennsylvania State University, University Park2

Abstract

Larger sperm reserve values were obtained when the saline used for homogenizing testes and epididymides included 0.05% Triton X-100. The amount of particulate matter which might obscure cells during hemacytometric counting was reduced. Twenty-two other rabbits injected with thymidine-3H were used to determine the time divisor necessary to calculate daily sperm production from testicular spermatid reserve data as obtained from testicular homogenates. The duration of one cycle of the seminiferous epithelium averaged 10.45 ±0.04 days, but for 9 of 20 rabbits there was an apparent difference, averaging 0.37±0.06 days, between testes. For rabbits, testicular spermatid reserves were concluded to represent 3.43 ±0.03 days production of spermatozoa. Sexually mature, New Zealand White rabbits produce about 210 x 106 spermatozoa per day.

Footnotes

1 Authorized for publication on April 16, 1968 as Paper No. 3398 in the Journal Series of the Pennsylvania Agricultural Experiment Station. This investigation was supported by Research Grant HD-01356-03 from the National Institute of Child Health and Human Development. Triton X-100 was kindly provided by Rhom and Haas, Philadelphia. Mrs. N. G. Borger and Mrs. B. R. Billeb provided skilled technical assistance.

2 Dairy Breeding Research Center, University Park, Pennsylvania, 16802.






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Copyright © 1969 by the American Society of Animal Science.