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Ultrasonic Treatment of Rabbit Testes¹

Cornell University, Ithaca, New York

Abstract

Ultrasonic waves were applied to one testis of each of 25 sexually mature Dutch-belted rabbits with the contralateral testis serving as a control. For all treatments transducers with the same frequency of 2.25 MHz and the same acoustic power of 1 mW/cm.² were used. Exposure time varied from 2 min. to 10 min. per location for each testis. The rabbits were sacrificed 1, 2 and 4 wk. after treatment. Testis size was unaffected by treatment, and there was no difference between treated and untreated sides. Diameter of the seminiferous tubules and spermatogenic activity, as indicated by the proportion of Stage 8 tubules, was the same in the control and treated testes. The percentages of motile, unstained and normal sperm ejaculated or recovered from different parts of the epididymides also were unaffected by ultrasonic radiation. Therefore, ultrasonic evaluation of the testis appears feasible without risk of injury to the tissue.

¹ Financial support by the Eastern Artificial Insemination Cooperative, Inc., is gratefully acknowledged.

² Department of Animal Science.