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Discussion

University of Pennsylvania, Philadelphia

Abstract

This morning Dr. Brinster brought to this audience a very thorough review of the factors which are important in the in vitro development of fertilized mammalian ova. Brinster has been one of the pioneer investigators in this area, and he reviewed for us the conditions which allowed him to take the mouse ovum from the two-cell stage on through the blastocyst in vitro. It is possible to take the mouse ovum from the one-cell to the two-cell stage, but in vitro systems do not allow complete development from the one-cell stage in that species, in the absence of some factor from the fallopian tube. Wittingham cultured mouse ova from the one-cell to the two-cell stage in vitro and then transferred them to fallopian tubes in organ culture and found that exposure to the oviducial environment for approximately 6 hr. was needed to allow development on through the blastocyst stage.