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Illinois Agriculture Experiment Station, Urbana
Abstract
Two experiments were conducted to evaluate the influence of dietary nitrogen source and level on gestation performance of rats exposed to cold. Diets with 5% protein, or 5% protein plus 10 essential amino acids (simulated casein pattern) or glutamic acid or both were fed. Pregnant rats were placed in a 7° or 23° C. environment on the day of mating and fed 18 and 12 gm., respectively. Nitrogen retention for each of five consecutive 4-day periods was determined.
At 23° C. supplementation of the basal diet with the essential amino acids increased weight gain. However, at 7° C. weight gain was not significantly affected. At both temperatures the amino acid mixture stimulated nitrogen retention during early gestation (first through fourth day).During the subsequent three 4-day periods, the amino acid mixture stimulated nitrogen retention at 23° C. However, at 7° C. during the same three periods, a positive response was not attained and the levels of nitrogen retained were only slightly above those from the unsupplemented diet at 23° C. The energy intake of the rats at 7° C. may have been inadequate to permit retention of the additional nitrogen consumed. In the final 4-day period (17th through 20th day) nitrogen retention from the amino acid supplemented diets at 7° was equal to that at 23° C. on the diet containing both essential amino acids and glutamic acid. Glutamic acid addition to the diet with essential amino acids increased nitrogen retention at 23° but not at 7° C.
1 Present address: Merck Institute for Therapeutic Research, Rahway, New Jersey.
2 This paper represents part of a thesis submitted by the senior author to the Graduate College of the University of Illinois in partial fulfillment of the requirements for the Ph.D.
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