J. Anim Sci.
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J. Anim Sci. 1968. 27:1357-1362.
© 1968 American Society of Animal Science
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Bacteriological Studies of Boar Semen1

F. A. Waltz, C. W. Foley, R. C. Herschler, L. W. Tiffany and B. J. Liska3

Purdue University,2, Lafayette, Indiana

Abstract

Seventy ejaculates of boar semen were collected from 13 mature boars with a previous history of satisfactory fertility. Microorganisms representing eight genera were isolated. Escherichia coli, Aerobacter sp., Proteus sp., Corynebacterium sp., Pseudomonas sp., Staphylococcus sp., and Bordetella sp., were found. No growth was obtained from 15 of the 70 collections. At least one sample from each of nine boars contained no bacterial colonies.

Three ejaculates from each of 13 boars were tested to determine the number of bacterial colonies arising from 1 ml. of semen. The mean number of colonies was 544 per ml. Statistical analysis indicated a significant (P<.05) difference between boars.

Among 16 different anti-bacterial agent sensivity discs tested, (37° C.) neomycin was the most effective and viomycin the least effective. When tested with stored (16° C.) extended semen, erythromycin, polymyxin B, neomycin, tylosin and sulfadiazine were the most effective in bacterial control. The motility scores were highest after storage in the presence of neomycin, polymyxin B and sulfadiazine.

Footnotes

1 Journal paper 2404, Purdue University Agricultural Experiment Station.

2 Department of Animal Sciences, School of Agriculture and the Departments of Veterinary Clinics and of Veterinary Microbiology, Pathology, and Public Health, School of Veterinary Science and Medicine.

3 The authors wish to acknowledge Mrs. Shirley Wolfe for statistical assistance.






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Copyright © 1968 by the American Society of Animal Science.