J. Anim Sci.
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J. Anim Sci. 1968. 27:1073-1080.
© 1968 American Society of Animal Science
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Effects of Urease Inhibitors on Sheep Fed Diets Containing Urea

A. J. Clifford1, J. R. Bourdette and A. D. Tillman2

Oklahoma Agricultural Experiment Station, Stillwater,3

Abstract

Barbituric acid, copper, and the nitrate ion were incorporated in urea-containing diets to determine their effects on ruminal urease activity and urea utilization by sheep and cattle. No inhibitors affected lamb growth in four different trials when these were included at three different levels in high or low grain diets. There appeared to be a trend toward reduced performance when the inhibitors were included.

A 2x4 factorial arrangement was used to study the effects of barbituric acid, copper, and the nitrate ion at levels of 0.01 %, 30 ppm, and 0.66%, respectively, when these were included in either high or low grain diets for sheep. None of the inhibitors affected (a) nitrogen digestibility or retention, (b) cellulose digestibility, (c) ruminal urease activity or (d) ruminal ammonia or urea levels.

In the sixth trial, fistulated steers were fed barbituric acid, copper and nitrates at levels of 0.01%, 30 ppm, and 0.66%, respectively, for 10 days, and samples were taken at 0, 30, 60, 90, 120 min. after feeding a high-roughage diet. All the inhibitors increased ruminal pH over that found in the controls. There were no consistent trends in ruminal urease activity, pH or concentration of nitrogen fractions with time on treatments.

In these trials none of the inhibitors had any beneficial effects upon urea utilization.

Footnotes

1 Present address: Department of Animal Science, Cornell University, Ithaca, New York.

2 Department of Animal Science.

3 Approved by the Director.






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Copyright © 1968 by the American Society of Animal Science.